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GI-1222 - ESCHERICHIA COLI REFERENCE LABORATORY (LREC)

Group info
Code: GI-1222
Short Name: LREC
Name: Escherichia coli Reference Laboratory (LREC)
Department: Departamento de Microbioloxía e Parasitoloxía
Web: http://www.usc.es/ecoli/
Contact: BLANCO ALVAREZ, JORGE - jorge.blanco@usc.es
Telf.

Research field
  • Spanish National Reference Laboratory for the Detection of E. coli in Food (AESAN, Ministerio de Sanidad).
  • Pathogenesis, epidemiology and microbiological diagnostic of E. coli strains that cause infections in humans and animals. STEC/VTEC, ETEC, EPEC, EIEC, EAggEC, AEEC, AIEC, ExPEC
  • Pathogenesis, diagnostic and molecular typing of verocytotoxigenic Escherichia coli (VTEC/STEC) O104:H4, O157:H7, O26:H11, O103:H2, O111:H8, O121:H19, O145:H- and other serotypes. Detection in clinical samples and food.
  • Intimin typing by PCR and DNA sequencing of eae gene of the LEE pathogenicity island.
  • Serotypes, virulence genes and molecular typing of extraintestinal pathogenic Escherichia coli (ExPEC).
  • Serotypes, virulence genes and molecular typing of E.coli producing extended-spectrum beta-lactamases (ESBL). Characterization of the intercontinental emergence clone O25:H4-ST131 producing CTX-M-15.
  • Molecular typing by PFGE and MLST.
  • Analytical methods for STEC/VTEC detection and other categories of diarrheagenic E. coli in foods (meat, dairy products and vegetables) and clinical samples. Detection of STEC/VTEC O104:H4, O157:H7 and others serotypes by conventional and real-time PCR.
  • E. coli vaccines.
Technology services
  • Detection of pathogenic E. coli in human and animalclinical samples. STEC/VTEC, EAEC, ETEC, EPEC, EIEC, AEEC, AIEC, and ExPEC
  • Detection of pathogenic E. coli in food. STEC/VTEC O104:H4, O157:H7.....
  • Detection of E.coli producing extended-spectrum beta-lactamases (ESBL). Characterization of the intercontinental emergence clone O25:H4-ST131 producing CTX-M-15
  • E. coli antisera. Antisera for serotyping of E. coli strains. O (O1-O181) and H (H1-H56) antisera for E. coli serotyping. Kits for E. coli serotyping: determination of O and H antigenes. Complete kit for E. coli serotyping with all available O and H antisera. Kit for serotyping human and animal pathogenic E. coli. Kits for serotyping: ExPEC (extraintestinal pathogenic E. coli, uropathogenic, septicemic and meningitis), VTEC/STEC (verotoxigenic or shiga-toxin producing E. coli) , EPEC (enteropathogenic E. coli), ETEC (enterotoxigenic E. coli), EIEC (enteroinvasive E. coli), EAEC (enteroaggregative E. coli), AIEC (adherent and invasive E. coli), NTEC (necrotoxigenic E. coli producing CNF1, CNF2 and CNF3). Kits for serotyping of extended-spectrum beta-lactamase (ESBL)-producing E. coli (ESBLEC) and other antibiotic resistant strains.
  • Molecular typing of E. coli by PFGE (pulsed-field gel electrophoresis) and MLST (multilocus sequence typing).
  • Kits for detection of virulence genes of E. coli by PCR
  • E. coli vaccines.
Key words
  • O antisera, H antisera, E. coli antisera, Bacteriology, BLEE, Clon ST131, E. coli, Escherichia coli, MLST, PCR, PFGE, Antimicrobial resistance, Bacterial toxins, Virulence genes, Vacunas
Researchers
Name Position ORCID Contact:
BLANCO ALVAREZ, JORGECoordinador0000-0003-0264-4136E-Mail: jorge.blanco@usc.es
BLANCO ALVAREZ, JESUS EULOGIOMembro0000-0002-5071-4760
BLANCO ALVAREZ, MIGUELMembro0000-0002-0572-0571
GARCIA MENENDEZ, VANESAMembro0000-0001-5459-8325
GARCIA RIESTRA, CARLOSMembro0000-0003-3584-5479
MORA GUTIERREZ, AZUCENAMembro0000-0002-0785-5795
DIAZ JIMENEZ, DAFNEIFP0000-0003-4621-2933
FLAMENT SIMON, SASKIA CAMILLEIFP0000-0003-0933-0850
LESTON CAMBEIRO, LUZIFP0000-0001-8518-8775
GARCIA MENIÑO, ISIDROPA0000-0001-9974-6571
IP Principal Investigator, IFP Training Research, PA Supporting Researchers and Technicians
External collaborators
Name Institution
Antonio Juárez Giménez  F. Biología, Universidad de Barcelona
Ricardo de la Fuente López  F. Veterinaria, Universidad Compluténse de Madrid
María Pilar Alonso García  Hospital Universitario Lucus Augusti
FERNANDO DE LA CRUZ CALAHORRA  Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria
MARÍA TERESA MUNIESA PÉREZ  Universitat de Barcelona
María del Rosario Rodicio Rodicio  Universidad de Oviedo
María de Toro Hernando  Centro de Investigación Biomédica de La Rioja (CIBIR)
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